Pedigree-based QTL analysis of flower size traits in two multi-parental diploid rose populations

Rose (Rosa spp.) is one of the most economically important ornamental species worldwide. Flower diameter, flower weight, and the number of petals and petaloids are key flower-size parameters and attractive targets for DNA-informed breeding. Pedigree-based analysis (PBA) using FlexQTL software was conducted using two sets of multi-parental diploid rose populations. Phenotypic data for flower diameter (Diam), flower weight (fresh (FWT)/dry (DWT)), number of petals (NP), and number of petaloids (PD) were collected over six environments (seasons) at two locations in Texas. The objectives of this study were to 1) identify new and/or validate previously reported QTL(s); 2) identify SNP haplotypes associated with QTL alleles (Q-/q-) of a trait and their sources; and 3) determine QTL genotypes for important rose breeding parents. Several new and previously reported QTLs for NP and Diam traits were identified. In addition, QTLs associated with flower weight and PD were identified for the first time. Two major QTLs with large effects were mapped for all traits. The first QTL was at the distal end of LG1 (60.44–60.95 Mbp) and was associated with Diam and DWT in the TX2WOB populations. The second QTL was consistently mapped in the middle region on LG3 (30.15–39.34 Mbp) and associated with NP, PD, and flower weight across two multi-parent populations (TX2WOB and TX2WSE). Haplotype results revealed a series of QTL alleles with differing effects at important loci for most traits. This work is distinct from previous studies by conducting co-factor analysis to account for the DOUBLE FLOWER locus while mapping QTL for NP. Sources of high-value (Q) alleles were identified, namely, ‘Old Blush’ and Rosa wichuraiana from J14-3 for Diam, while ‘Violette’ and PP-J14-3 were sources for other traits. In addition, the source of the low-value (q) alleles for Diam was ‘Little Chief’, and Rosa wichuraiana through J14-3 was the source for the remaining traits. Hence, our results can potentially inform parental/seedling selections as means to improve ornamental quality in roses and a step towards implementing DNA-informed techniques for use in rose breeding programs

Experimental and Numerical Study on the Performance of Double Membrane Wing for Long-Endurance Low-Speed Aircraft

Flexible membrane structure is generally used as wing skin for long-endurance low-speed aircraft, such as solar aircraft, to control the structure weight within the allowable range. Predictably, the elastic deformation of the membrane under complex loads will cause uncertain impacts on the aerodynamic performance. The existing research indicates that the deformation of the membrane wing is helpful to improve the aerodynamic characteristics. However, most of the research objects are non-thickness membrane wings. In this paper, wind tunnel experiments are performed on double membrane wings. The experiment results indicate that the membrane deformation behavior is related to the surface curvature distribution and will change the camber and thickness of the airfoil. The deformation has little effect on lift but has a significant effect on drag and pitching moment. On this basis, a high-precision fluid structure coupling analysis method for the wider range of research is introduced. The numerical analysis indicates that the deformation can delay the stall angle by 1°. Furthermore, based on the numerical results, suggestions on prestress setting during membrane skin laying are provided, and the numerical simulation results of two flexible skin wings are compared. The research results of this paper provide a scientific basis for the aerodynamic design and analysis of long-endurance low-speed aircraft

Experimental and Numerical Study on the Performance of Double Membrane Wing for Long-Endurance Low-Speed Aircraft

Flexible membrane structure is generally used as wing skin for long-endurance low-speed aircraft, such as solar aircraft, to control the structure weight within the allowable range. Predictably, the elastic deformation of the membrane under complex loads will cause uncertain impacts on the aerodynamic performance. The existing research indicates that the deformation of the membrane wing is helpful to improve the aerodynamic characteristics. However, most of the research objects are non-thickness membrane wings. In this paper, wind tunnel experiments are performed on double membrane wings. The experiment results indicate that the membrane deformation behavior is related to the surface curvature distribution and will change the camber and thickness of the airfoil. The deformation has little effect on lift but has a significant effect on drag and pitching moment. On this basis, a high-precision fluid structure coupling analysis method for the wider range of research is introduced. The numerical analysis indicates that the deformation can delay the stall angle by 1°. Furthermore, based on the numerical results, suggestions on prestress setting during membrane skin laying are provided, and the numerical simulation results of two flexible skin wings are compared. The research results of this paper provide a scientific basis for the aerodynamic design and analysis of long-endurance low-speed aircraft

Capturing the Long-Sought Dy@<i>C_2v</i>(5)-C₈₀ via Benzyl Radical Stabilization

Endohedral metallofullerenes (EMFs) are one type of intriguing metal/carbon hybrid molecule with the molecule configuration of sphere cavity-encapsulating metal ions/metal clusters due to their unique physicochemical properties and corresponding application in the fields of biological materials, single molecule magnet materials and energy conversion materials. Although the EMF family is growing, and versatile EMFs have been successfully synthesized and confirmed using crystal structures, some expected EMF members have not been observed using the conventional fullerene separation and purify strategy. These missing EMFs raise an interesting scientific issue as to whether this is due to the difficulty in separating them from the in situ formed carbon soot. Herein, we successfully captured a long-sought dysprosium-based EMF bearing a C2v(5)-C80 cage (Dy@C2v(5)-C80) in the form of Dy@C2v(5)-C80(CH2Ph)(Ph = −C6H5) from carbon soot containing versatile EMFs using simple benzyl radical functionalization and unambiguously confirmed the molecule structure using single crystal X-ray diffraction characterization. Meanwhile, the crystal structure of Dy@C2v(5)-C80(CH2Ph) showed that a single benzyl group was grafted onto the (5,6,6)-carbon, suggesting the open-shell electronic configuration of Dy@C2v(5)-C80. The theoretical calculations unveiled that the benzyl radical addition enables the modulation of the electronic configuration of Dy@C2v(5)-C80 and the corresponding stabilization of Dy@C2v(5)-C80 in conventional organic solvents. This facile stabilization strategy via benzyl radical addition exhibits the considerable capability to capture these missing EMFs, with the benefit of enriching the endohedral fullerene family

EspF of Enterohemorrhagic Escherichia coli Enhances Apoptosis via Endoplasmic Reticulum Stress in Intestinal Epithelial Cells: An Isobaric Tags for Relative and Absolute Quantitation-Based Comparative Proteomic Analysis.

There have been large foodborne outbreaks related to Enterohemorrhagic Escherichia coli (EHEC) around the world. Among its virulence proteins, the EspF encoded by locus of enterocyte effacement is one of the most known functional effector proteins. In this research, we infected the HT-29 cells with the EHEC wild type strain and EspF-deficient EHEC strain. Via the emerging technique isobaric tags for relative and absolute quantitation (iTRAQ), we explored the pathogenic characteristics of EspF within host cells. Our data showed that the differences regarding cellular responses mainly contained immune regulation, protein synthesis, signal transduction, cellular assembly and organization, endoplasmic reticulum (ER) stress, and apoptosis. Notably, compared with the EspF-deficient strain, the protein processing in the ER and ribosome were upregulated during wild type (WT) infection. Our findings proved that the EspF of Enterohemorrhagic Escherichia coli induced ER stress in intestinal epithelial cells; the ER stress-dependent apoptosis pathway was also activated within the host cells. This study provides insight into the virulence mechanism of protein EspF, which will deepen our general understanding of A/E pathogens and their interaction with host proteins